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验证数据展示

  • IHC staining of human tonsillitis using Biotin-66408

    IHC staining of human tonsillitis using Biotin-66408

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-66408 (IGHG4 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Human IgG4 antibody included
  • IHC staining of human tonsillitis using Biotin-66408

    IHC staining of human tonsillitis using Biotin-66408

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-66408 (IGHG4 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Human IgG4 antibody included

Biotin-conjugated IgG4 Monoclonal antibody

Human IgG4 Monoclonal Antibody for IHC
Cat No. Biotin-66408
CloneNo. 1A1A2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC

Synonyms

IgG4, IGHG4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Biotin
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Human IgG4 Ready-To-Use IHC Kit. Human IgG4 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-66408 targets Human IgG4 in IHC applications and shows reactivity with human samples.

Tested Applications IHC Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name immunoglobulin heavy constant gamma 4 (G4m marker)
Synonyms IgG4, IGHG4
Calculated Molecular Weight 55 kDa
GenBank Accession NumberAL928742
Gene Symbol IGHG4
Gene ID (NCBI) 3503
RRIDAB_2883071
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4. This antibody is Biotin conjugated.

实验方案

Product Specific Protocols
IHC protocol for Biotin Human IgG4 antibody Biotin-66408Download protocol
Standard Protocols
Click here to view our Standard Protocols