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验证数据展示

  • IHC staining of human tonsillitis using Biotin-66408

    IHC staining of human tonsillitis using Biotin-66408

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-66408 (IGHG4 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Human IgG4 antibody included
  • IHC staining of human tonsillitis using Biotin-66408

    IHC staining of human tonsillitis using Biotin-66408

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-66408 (IGHG4 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Human IgG4 antibody included

Biotin-conjugated IgG4 Monoclonal antibody

Human IgG4 Monoclonal Antibody for IHC
Cat No. Biotin-66408

产品说明书

CloneNo. 1A1A2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IHC

别名

IgG4, IGHG4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Biotin
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Human IgG4 Ready-To-Use IHC Kit. Human IgG4 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-66408 targets Human IgG4 in IHC applications and shows reactivity with human samples.

经测试应用 IHC Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 immunoglobulin heavy constant gamma 4 (G4m marker)
别名 IgG4, IGHG4
计算分子量 55 kDa
GenBank蛋白编号AL928742
基因名称 IGHG4
Gene ID (NCBI) 3503
RRIDAB_2883071
偶联类型 Biotin
形式 Liquid
纯化方式Protein G purification
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4. This antibody is Biotin conjugated.

实验方案

Product Specific Protocols
IHC protocol for Biotin Human IgG4 antibody Biotin-66408Download protocol
Standard Protocols
Click here to view our Standard Protocols