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IGF2 Recombinant antibody

IGF2 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241746D3

Cat no : 84643-1-RR

Print datasheet

Synonyms

proIGF-II, PP1446, Insulin-like growth factor II Ala-25 Del, IGF-II, IGF II

验证数据展示

  • WB analysis of HuH-7 using 84643-1-RR

    WB analysis of HuH-7 using 84643-1-RR

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 84643-1-RR (IGF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84643-1-RR

    Affinity and Kinetic Characterization of 84643-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84643-1-RR against Human IGF2 were performed. The affinity constant is 0.159 nM.

经过测试的应用

Positive WB detected inHuH-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84643-1-RR is IGF2 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen IGF2 fusion protein HZ-1161 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name insulin-like growth factor 2 (somatomedin A)
Synonyms proIGF-II, PP1446, Insulin-like growth factor II Ala-25 Del, IGF-II, IGF II
Observed Molecular Weight20 kDa
Gene Symbol IGF2
Gene ID (NCBI) 3481
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01344
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Insulin-like growth factor 2 (IGF2) is a crucial protein that plays a significant role in regulating growth, particularly during normal fetal development, and is also often dysregulated during tumorigenesis. IGF2 is one of the main ligands of the type 1 IGF receptor (IGF-1R), a tyrosine kinase receptor that transmits signals upon binding with IGF-II. IGF2 is predominantly secreted via the placenta during pregnancy and, after birth, primarily by hepatocytes, with its secretion being independent of growth hormone (GH) secretion, unlike IGF-I.

实验方案

Product Specific Protocols
WB protocol for IGF2 antibody 84643-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols