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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human pro IGF-II protein

Activity

12-80 ng/mL

Species Reactivity

human,mouse

Purity

>95%

Cat No : HZ-1161

Print datasheet

Synonyms

C11orf43, FLJ22066, FLJ44734, IGF II, IGF2, INSIGF, Insulin like growth factor II, pp9974, Somatomedin A

Validation Data Gallery

  • Purity of recombinant human Pro-IGF-II was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human Pro-IGF-II was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • The activity was determined by the dose-dependent stimulation of the proliferation of MCF-7 cells (human breast cancer cell line) using the Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

    The activity was determined by the dose-dependent stimulation of the proliferation of MCF-7 cells (human breast cancer cell line) using the Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

Technical Specifications

GeneID 3481
Species Human
Expression HEK293
Activity 12-80 ng/mL
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP01344
Molecular Mass 25 kDa reduced and non-reduced, monomer, glycosylated
Formulation 10 mM Tris-HCl pH 7.4 + 350 mM NaCl, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

IGF2 is a hormone involved in the regulation of cell proliferation, growth, migration, differentiation, and survival. IGF2 is mostly expressed in early embryonic and fetal development. Adult IGF2 expression occurs in the liver and epithelial cells lining the surface of the brain. IGF2 can also be detected in plasma (PMID: 24593700; 23257688)

Synonyms

C11orf43, FLJ22066, FLJ44734, IGF II, IGF2, INSIGF, Insulin like growth factor II, pp9974, Somatomedin A

Publications

SpeciesTitle
mouse

Stem Cells

Pro-Insulin-Like Growth Factor-II Ameliorates Age-Related Inefficient Regenerative Response by Orchestrating Self-Reinforcement Mechanism of Muscle Regeneration.

Authors - Madoka Ikemoto-Uezumi
View Article
human

J Clin Endocrinol Metab

Paracrine Pathways in Uterine Leiomyoma Stem Cells Involve Insulinlike Growth Factor 2 and Insulin Receptor A.

Authors - Molly B Moravek
View Article