验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | LPS and Brefeldin A treated human PBMCs |
For other applications, we recommend the unconjugated version of this antibody, 65277-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL405-65277 targets IL-12A/IL-12 p35 in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 |
Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) |
| 别名 | IL-12, 1E12H6, CLMF, CLMF p35, IL 12 subunit p35 |
| 计算分子量 | 25 kDa |
| GenBank蛋白编号 | BC104982 |
| 基因名称 | IL-12A |
| Gene ID (NCBI) | 3592 |
| RRID | AB_3083856 |
| 偶联类型 | CoraLite® Plus 405 Fluorescent Dye |
| 最大激发/发射波长 | 399 nm / 422 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P29459 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 405 IL-12A/IL-12 p35 antibody CL405-65277 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
