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验证数据展示

  • FC experiment of human PBMCs using CL647-65277

    FC experiment of human PBMCs using CL647-65277

    1X10^6 LPS and Brefeldin A treated human PBMCs were surface stained with CoraLite® Plus 555 Anti-Human HLA-DR then intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human IL-12 (CL647-65277, Clone:1E12H6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

  • FC experiment of human PBMCs using CL647-65277

    FC experiment of human PBMCs using CL647-65277

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human IL-12 (CL647-65277, Clone:1E12H6) (red) or Mouse IgG1 Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human IL-12 (CL647-65277, Clone:1E12H6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

CoraLite® Plus 647 Anti-Human IL-12 (1E12H6)

IL-12 Monoclonal Antibody for FC (Intra)
Cat No. CL647-65277

产品说明书

CloneNo. 1E12H6

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

FC (Intra)

别名

IL-12, IL-12 p35, IL-12 subunit p35, IL12A, IL-12A, Interleukin 12 subunit alpha, Interleukin-12 subunit alpha, NFSK, NKSF1, P35, 1E12H6, CLMF, CLMF p35, IL 12 subunit p35, IL 12A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inLPS and Brefeldin A treated human PBMCs
Positive FC detected inLPS and Brefeldin A treated human PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65277-1-Ig

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65277 targets IL-12 in FC (Intra) applications and shows reactivity with Human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human
免疫原 IL-12 fusion protein HZ-1256 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
别名 IL-12, IL-12 p35, IL-12 subunit p35, IL12A, IL-12A, Interleukin 12 subunit alpha, Interleukin-12 subunit alpha, NFSK, NKSF1, P35, 1E12H6, CLMF, CLMF p35, IL 12 subunit p35, IL 12A
计算分子量 25 kDa
GenBank蛋白编号BC104982
基因名称 IL-12A
Gene ID (NCBI) 3592
RRIDAB_3084817
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 IL-12 antibody CL647-65277Download protocol
Standard Protocols
Click here to view our Standard Protocols