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验证数据展示

  • FC experiment of human PBMCs using CL647-65277

    FC experiment of human PBMCs using CL647-65277

    1X10^6 LPS and Brefeldin A treated human PBMCs were surface stained with CoraLite® Plus 555 Anti-Human HLA-DR then intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human IL-12 (CL647-65277, Clone:1E12H6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

  • FC experiment of human PBMCs using CL647-65277

    FC experiment of human PBMCs using CL647-65277

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human IL-12 (CL647-65277, Clone:1E12H6) (red) or Mouse IgG1 Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human IL-12 (CL647-65277, Clone:1E12H6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

CoraLite® Plus 647 Anti-Human IL-12 (1E12H6)

IL-12 Monoclonal Antibody for FC (Intra)
Cat No. CL647-65277

Print datasheet

CloneNo. 1E12H6

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Synonyms

IL-12, IL-12 p35, IL-12 subunit p35, IL12A, IL-12A, Interleukin 12 subunit alpha, Interleukin-12 subunit alpha, NFSK, NKSF1, P35, 1E12H6, CLMF, CLMF p35, IL 12 subunit p35, IL 12A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inLPS and Brefeldin A treated human PBMCs
Positive FC detected inLPS and Brefeldin A treated human PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65277 targets IL-12 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human
Immunogen IL-12 fusion protein HZ-1256 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
Synonyms IL-12, IL-12 p35, IL-12 subunit p35, IL12A, IL-12A, Interleukin 12 subunit alpha, Interleukin-12 subunit alpha, NFSK, NKSF1, P35, 1E12H6, CLMF, CLMF p35, IL 12 subunit p35, IL 12A
Calculated Molecular Weight 25 kDa
GenBank Accession NumberBC104982
Gene Symbol IL-12A
Gene ID (NCBI) 3592
RRIDAB_3084817
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 IL-12 antibody CL647-65277Download protocol
Standard Protocols
Click here to view our Standard Protocols