验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | LPS and Brefeldin A treated human PBMCs |
| Positive FC detected in | LPS and Brefeldin A treated human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
FITC-65277 targets IL-12 in FC (Intra) applications and shows reactivity with Human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | Human |
| 免疫原 | IL-12 fusion protein HZ-1256 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) |
| 别名 | IL-12, IL-12 p35, IL-12 subunit p35, IL12A, IL-12A, Interleukin 12 subunit alpha, Interleukin-12 subunit alpha, NFSK, NKSF1, P35, 1E12H6, CLMF, CLMF p35, IL 12 subunit p35, IL 12A |
| 计算分子量 | 25 kDa |
| GenBank蛋白编号 | BC104982 |
| 基因名称 | IL-12A |
| Gene ID (NCBI) | 3592 |
| RRID | AB_3085007 |
| 偶联类型 | FITC Plus Fluorescent Dye |
| 最大激发/发射波长 | 495 nm / 524 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus IL-12 antibody FITC-65277 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |