- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated Integrin Alpha 4 Polyclonal antibody
Integrin Alpha 4 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive FC detected in | Jurkat cells |
For other applications, we recommend the unconjugated version of this antibody, 19676-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-19676 targets Integrin Alpha 4 in FC (Intra) applications and shows reactivity with human samples.
| Tested Applications | FC (Intra) |
| Tested Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) |
| Synonyms | CD49D, IA4, Integrin alpha 4, Integrin alpha IV, ITGA4, VLA 4 subunit alpha |
| Calculated Molecular Weight | 115 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | NM_000885 |
| Gene Symbol | ITGA4 |
| Gene ID (NCBI) | 3676 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13612 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ITGA4, also named as CD49D, belongs to the integrin alpha chain family. ITGA4 is a receptor for fibronectin, VCAM1 and MADCAM1. ITGA4 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. It recognizes the sequence Q-I-D-S in VCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells ITGA4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 Integrin Alpha 4 antibody CL488-19676 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
