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CoraLite® Plus 488-conjugated LDHB Polyclonal antibody
LDHB Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy LDHB Ready-To-Use IHC Kit. LDHB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14824-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-14824 targets LDHB in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | LDHB fusion protein Ag6605 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | lactate dehydrogenase B |
Synonyms | lactate dehydrogenase B, EC:1.1.1.27, LDHA, LDH H, LDH B |
Calculated Molecular Weight | 36 kDa |
Observed Molecular Weight | 35 kDa |
GenBank Accession Number | BC002362 |
Gene Symbol | LDHB |
Gene ID (NCBI) | 3945 |
RRID | AB_2919103 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P07195 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of pyruvate and NADH to lactate and NAD+ in the glycolytic pathway (PMID: 26269128). It is glycolytic enzymes and it is positively regulated by mTOR.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 LDHB antibody CL488-14824 | Download protocol |
FC protocol for CL Plus 488 LDHB antibody CL488-14824 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |