验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy LDHB Ready-To-Use IHC Kit. LDHB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14824-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-14824 targets LDHB in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | LDHB fusion protein Ag6605 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lactate dehydrogenase B |
| 别名 | lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, TRG 5 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC002362 |
| 基因名称 | LDHB |
| Gene ID (NCBI) | 3945 |
| RRID | AB_2919620 |
| 偶联类型 | CoraLite®555 Fluorescent Dye |
| 最大激发/发射波长 | 557 nm / 570nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P07195 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of pyruvate and NADH to lactate and NAD+ in the glycolytic pathway (PMID: 26269128). It is glycolytic enzymes and it is positively regulated by mTOR.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL555 LDHB antibody CL555-14824 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |