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验证数据展示

  • FC experiment of HeLa using CL647-14824

    FC experiment of HeLa using CL647-14824

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human LDHB (CL647-14824) (red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL647-14824

    IF Staining of HeLa using CL647-14824

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 647 LDHB antibody (CL647-14824) at dilution of 1:100.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated LDHB Polyclonal antibody

LDHB Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-14824

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

lactate dehydrogenase B, EC:1.1.1.27, LDHA, LDH H, LDH B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LDHB Ready-To-Use IHC Kit. LDHB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14824-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-14824 targets LDHB in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 LDHB fusion protein Ag6605 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lactate dehydrogenase B
别名 lactate dehydrogenase B, EC:1.1.1.27, LDHA, LDH H, LDH B
计算分子量 36 kDa
观测分子量 35 kDa
GenBank蛋白编号BC002362
基因名称 LDHB
Gene ID (NCBI) 3945
RRIDAB_2934904
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07195
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of pyruvate and NADH to lactate and NAD+ in the glycolytic pathway (PMID: 26269128). It is glycolytic enzymes and it is positively regulated by mTOR.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 LDHB antibody CL647-14824Download protocol
FC protocol for CL Plus 647 LDHB antibody CL647-14824Download protocol
Standard Protocols
Click here to view our Standard Protocols