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Lamin B2 Recombinant antibody

Lamin B2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230184G4

Cat no : 82801-3-RR

Print datasheet

Synonyms

LMNB2, LMN2, Lamin-B2, LAMB2, 230184G4

验证数据展示

  • IF Staining of HepG2 using 82801-3-RR

    IF Staining of HepG2 using 82801-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B2 antibody (82801-3-RR, Clone: 230184G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • FC experiment of HepG2 using 82801-3-RR

    FC experiment of HepG2 using 82801-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Lamin B2 Recombinant antibody (82801-3-RR, Clone:230184G4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82801-3-RR

    Affinity and Kinetic Characterization of 82801-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82801-3-RR against Human Lamin B2 were performed. The affinity constant is 0.428 nM.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy Lamin B2 Ready-To-Use IHC Kit. Lamin B2 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82801-3-RR targets Lamin B2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Lamin B2 fusion protein Ag1335 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lamin B2
Synonyms LMNB2, LMN2, Lamin-B2, LAMB2, 230184G4
Calculated Molecular Weight 68 kDa
GenBank Accession NumberBC006551
Gene Symbol Lamin B2
Gene ID (NCBI) 84823
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ03252
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).

实验方案

Product Specific Protocols
IF protocol for Lamin B2 antibody 82801-3-RRDownload protocol
FC protocol for Lamin B2 antibody 82801-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols