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验证数据展示

  • IF Staining of HepG2 using CL488-12400

    IF Staining of HepG2 using CL488-12400

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 MAGEB1 antibody (CL488-12400) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HepG2 using CL488-12400

    FC experiment of HepG2 using CL488-12400

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Mageb1 Polyclonal Antibody (CL488-12400)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated MAGEB1 Polyclonal antibody

MAGEB1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-12400

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

MAGE Xp, MAGE B1 antigen, DSS-AHC critical interval MAGE superfamily 10, DAM10, CT3.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 12400-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12400 targets MAGEB1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 MAGEB1 fusion protein Ag3094 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 melanoma antigen family B, 1
别名 MAGE Xp, MAGE B1 antigen, DSS-AHC critical interval MAGE superfamily 10, DAM10, CT3.1
计算分子量 347 aa, 39 kDa
观测分子量 43-45 kDa
GenBank蛋白编号BC013772
基因名称 MAGEB1
Gene ID (NCBI) 4112
RRIDAB_3672539
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43366
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGEB1, also named as MAGEL1, MAGEXP, DAM10 and CT3.1, is a member of the MAGEB gene family. MAGE genes encode for tumor-rejection antigens and are expressed in tumors of different histologic types and Testis/placenta, but not in other normal tissues. The MW of MAGEB1 is about 40-45kd.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MAGEB1 antibody CL488-12400Download protocol
FC protocol for CL Plus 488 MAGEB1 antibody CL488-12400Download protocol
Standard Protocols
Click here to view our Standard Protocols