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验证数据展示

  • IF Staining of SH-SY5Y using 84306-4-RR (same clone as 84306-4-PBS)

    IF Staining of SH-SY5Y using 84306-4-RR (same clone as 84306-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using MAP2 antibody (84306-4-RR, Clone: 241653H8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84306-4-PBS in a different storage buffer formulation.

  • WB analysis using 84306-4-RR (same clone as 84306-4-PBS)

    WB analysis using 84306-4-RR (same clone as 84306-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84306-4-RR (MAP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84306-4-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 84306-4-RR (same clone as 84306-4-PBS)

    IF Staining of mouse brain using 84306-4-RR (same clone as 84306-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using MAP2 antibody (84306-4-RR, Clone: 241653H8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84306-4-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 84306-4-RR (same clone as 84306-4-PBS)

    IF Staining of rat brain using 84306-4-RR (same clone as 84306-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using MAP2 antibody (84306-4-RR, Clone: 241653H8,red ) at dilution of 1:210 and FlexAble CoraLite® Plus 594 Antibody Labeling Kit for Rabbit IgG(KFA009), GFAP antibody (81063-1-RR, Clone: 4C6, green) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84306-4-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 84306-4-RR (same clone as 84306-4-PBS)

    IF Staining of mouse brain using 84306-4-RR (same clone as 84306-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using MAP2 antibody (84306-4-RR, Clone: 241653H8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84306-4-PBS in a different storage buffer formulation.

MAP2 Recombinant antibody, PBS Only

MAP2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IF-P, IF-Fro, Indirect ELISA
Cat No. 84306-4-PBS

产品说明书

CloneNo. 241653H8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IF-P, IF-Fro, Indirect ELISA

别名

MAP2C, MAP2B, MAP2A, MAP-2, MAP 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MAP2 Ready-To-Use IHC Kit. MAP2 primary antibody included.

产品信息

84306-4-PBS targets MAP2 in WB, IF/ICC, IF-P, IF-Fro, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IF-P, IF-Fro, Indirect ELISA Application Description
经测试反应性 human
免疫原 MAP2 fusion protein Ag11580 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 microtubule-associated protein 2
别名 MAP2C, MAP2B, MAP2A, MAP-2, MAP 2
计算分子量 200 kDa
观测分子量280-300 kDa, 70-85 kDa
GenBank蛋白编号BC038857
基因名称 MAP2
Gene ID (NCBI) 4133
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP11137
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in the brain and has an important role in neuronal morphogenesis. Multiple high MW and low MW MAP2 isoforms are expressed within the proximal segment of axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally-specific manner. The 280 kDa MAP2B is present throughout rat brain development, and the slightly larger MAP2A appears first during the end of the second week of postnatal life. MAP2C, composed of several bands of about 70 kDa, is present during early brain development and largely disappears from the mature brain except for the retina, olfactory bulb, and cerebellum. In addition, some isoforms with lower MW around 50-60 kDa also exist. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.