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验证数据展示

  • IF Staining of SH-SY5Y using 84306-4-RR

    IF Staining of SH-SY5Y using 84306-4-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using MAP2 antibody (84306-4-RR, Clone: 241653H8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • WB analysis using 84306-4-RR

    WB analysis using 84306-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84306-4-RR (MAP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 84306-4-RR

    IF Staining of mouse brain using 84306-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using MAP2 antibody (84306-4-RR, Clone: 241653H8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 84306-4-RR

    IF Staining of rat brain using 84306-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using MAP2 antibody (84306-4-RR, Clone: 241653H8,red ) at dilution of 1:210 and FlexAble CoraLite® Plus 594 Antibody Labeling Kit for Rabbit IgG(KFA009), GFAP antibody (81063-1-RR, Clone: 4C6, green) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 84306-4-RR

    IF Staining of mouse brain using 84306-4-RR

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using MAP2 antibody (84306-4-RR, Clone: 241653H8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

MAP2 Recombinant antibody

MAP2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IF-P, IF-Fro, ELISA
Cat No. 84306-4-RR

Print datasheet

CloneNo. 241653H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IF-P, IF-Fro, ELISA

Synonyms

MAP2C, MAP2B, MAP2A, MAP-2, MAP 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, U-251 cells
Positive IF-P detected inrat brain tissue, mouse brain tissue
Positive IF-Fro detected inmouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy MAP2 Ready-To-Use IHC Kit. MAP2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:105-1:420
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84306-4-RR targets MAP2 in WB, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, IF-P, IF-Fro, ELISA Application Description
Tested Reactivity human
Immunogen MAP2 fusion protein Ag11580 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name microtubule-associated protein 2
Synonyms MAP2C, MAP2B, MAP2A, MAP-2, MAP 2
Calculated Molecular Weight 200 kDa
Observed Molecular Weight280-300 kDa, 70-85 kDa
GenBank Accession NumberBC038857
Gene Symbol MAP2
Gene ID (NCBI) 4133
RRIDAB_3671847
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP11137
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in the brain and has an important role in neuronal morphogenesis. Multiple high MW and low MW MAP2 isoforms are expressed within the proximal segment of axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally-specific manner. The 280 kDa MAP2B is present throughout rat brain development, and the slightly larger MAP2A appears first during the end of the second week of postnatal life. MAP2C, composed of several bands of about 70 kDa, is present during early brain development and largely disappears from the mature brain except for the retina, olfactory bulb, and cerebellum. In addition, some isoforms with lower MW around 50-60 kDa also exist. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.

实验方案

Product Specific Protocols
WB protocol for MAP2 antibody 84306-4-RRDownload protocol
IF protocol for MAP2 antibody 84306-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols