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验证数据展示

  • FC experiment of HeLa using CL488-82929-2

    FC experiment of HeLa using CL488-82929-2

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Mapkapk2 Recombinant Antibody (CL488-82929-2, Clone:230197D6)(red), or 0.25 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated MAPKAPK2 Recombinant antibody

MAPKAPK2 Recombinant Antibody for FC (Intra)
Cat No. CL488-82929-2

产品说明书

CloneNo. 230197D6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

MAPKAPK 2, MAPKAP kinase 2, MAPK-activated protein kinase 2, MAP kinase-activated protein kinase 2, EC:2.7.11.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MAPKAPK2 Ready-To-Use IHC Kit. MAPKAPK2 primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82929-2 targets MAPKAPK2 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 MAPKAPK2 fusion protein Ag34196 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitogen-activated protein kinase-activated protein kinase 2
别名 MAPKAPK 2, MAPKAP kinase 2, MAPK-activated protein kinase 2, MAP kinase-activated protein kinase 2, EC:2.7.11.1
计算分子量 400 aa, 46 kDa
观测分子量42-46 kDa
GenBank蛋白编号BC036060
基因名称 MAPKAPK2
Gene ID (NCBI) 9261
RRIDAB_3673139
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49137
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 MAPKAPK2 antibody CL488-82929-2Download protocol
Standard Protocols
Click here to view our Standard Protocols