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CoraLite® Plus 488-conjugated MAPKAPK2 Recombinant antibody

MAPKAPK2 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230197D6

Cat no : CL488-82929-2

Print datasheet

Synonyms

MAPKAPK 2, MAPKAP kinase 2, MAPK-activated protein kinase 2, MAP kinase-activated protein kinase 2, EC:2.7.11.1

验证数据展示

  • FC experiment of HeLa using CL488-82929-2

    FC experiment of HeLa using CL488-82929-2

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Mapkapk2 Recombinant Antibody (CL488-82929-2, Clone:230197D6)(red), or 0.25 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MAPKAPK2 Ready-To-Use IHC Kit. MAPKAPK2 primary antibody included.

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-82929-2 is MAPKAPK2 Fusion Protein expressed in E. coli.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen MAPKAPK2 fusion protein Ag34196 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitogen-activated protein kinase-activated protein kinase 2
Synonyms MAPKAPK 2, MAPKAP kinase 2, MAPK-activated protein kinase 2, MAP kinase-activated protein kinase 2, EC:2.7.11.1
Calculated Molecular Weight 400 aa, 46 kDa
Observed Molecular Weight42-46 kDa
GenBank Accession NumberBC036060
Gene Symbol MAPKAPK2
Gene ID (NCBI) 9261
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49137
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 MAPKAPK2 antibody CL488-82929-2Download protocol
Standard Protocols
Click here to view our Standard Protocols