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验证数据展示

  • IF Staining of HeLa using CL488-81910

    IF Staining of HeLa using CL488-81910

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 MAVS; VISA antibody (CL488-81910, Clone: 7F14 ) at dilution of 1:100.

  • FC experiment of U2OS using CL488-81910

    FC experiment of U2OS using CL488-81910

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated MAVS; VISA Recombinant antibody (CL488-81910, Clone:7F14)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated MAVS; VISA Recombinant antibody

MAVS; VISA Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-81910

产品说明书

CloneNo. 7F14

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

MAVS, KIAA1271, IPS-1, IPS1, IPS 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy MAVS Ready-To-Use IHC Kit. MAVS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81910-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-81910 targets MAVS; VISA in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 MAVS; VISA fusion protein Ag5655 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitochondrial antiviral signaling protein
别名 MAVS, KIAA1271, IPS-1, IPS1, IPS 1
计算分子量 57 kDa
观测分子量75 kDa
GenBank蛋白编号BC044952
基因名称 MAVS
Gene ID (NCBI) 57506
RRIDAB_3084508
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z434
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN-β promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MAVS; VISA antibody CL488-81910Download protocol
Standard Protocols
Click here to view our Standard Protocols