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CoraLite® Plus 488-conjugated MAVS; VISA Recombinant antibody

MAVS; VISA Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

7F14

Cat no : CL488-81910

Print datasheet

Synonyms

MAVS, KIAA1271, IPS-1, IPS1, IPS 1

验证数据展示

  • IF Staining of HeLa using CL488-81910

    IF Staining of HeLa using CL488-81910

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 MAVS; VISA antibody (CL488-81910, Clone: 7F14 ) at dilution of 1:100.

  • FC experiment of U2OS using CL488-81910

    FC experiment of U2OS using CL488-81910

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated MAVS; VISA Recombinant antibody (CL488-81910, Clone:7F14)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 81910-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-81910 targets MAVS; VISA in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen MAVS; VISA fusion protein Ag5655 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitochondrial antiviral signaling protein
Synonyms MAVS, KIAA1271, IPS-1, IPS1, IPS 1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC044952
Gene Symbol MAVS
Gene ID (NCBI) 57506
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7Z434
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN-β promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MAVS; VISA antibody CL488-81910Download protocol
Standard Protocols
Click here to view our Standard Protocols