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Biotin-conjugated MAdCAM1 Monoclonal antibody

MAdCAM1 Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IHC

Conjugate

Biotin

CloneNo.

2A12E8

Cat no : Biotin-66594

Print datasheet

Synonyms

hMAdCAM 1, MACAM1, MAdCAM 1, MADCAM1

验证数据展示

  • IHC staining of human small intestine using Biotin-66594

    IHC staining of human small intestine using Biotin-66594

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using Biotin-66594 (MAdCAM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAdCAM1 antibody included
  • IHC staining of human small intestine using Biotin-66594

    IHC staining of human small intestine using Biotin-66594

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using Biotin-66594 (MAdCAM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAdCAM1 antibody included

经过测试的应用

Positive IHC detected inhuman small intestine tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MAdCAM1 Ready-To-Use IHC Kit. MAdCAM1 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-66594 targets MAdCAM1 in IHC applications and shows reactivity with Human samples.

Tested Applications IHC Application Description
Tested Reactivity Human
Immunogen MAdCAM1 fusion protein Ag16184 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name mucosal vascular addressin cell adhesion molecule 1
Synonyms hMAdCAM 1, MACAM1, MAdCAM 1, MADCAM1
Calculated Molecular Weight 406 aa, 43 kDa
GenBank Accession NumberBC142629
Gene Symbol MADCAM1
Gene ID (NCBI) 8174
RRIDAB_2918948
Conjugate Biotin
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13477
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.

实验方案

Product Specific Protocols
IHC protocol for Biotin MAdCAM1 antibody Biotin-66594Download protocol
Standard Protocols
Click here to view our Standard Protocols