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CoraLite®594-conjugated Myosin Light Chain 2 Monoclonal antibody

Myosin Light Chain 2 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5A1F1

Cat no : CL594-60229

Print datasheet

Synonyms

MLC 2v, MYL2, MLC-2s/v, MLC-2, MLC2

验证数据展示

  • IF Staining of C2C12 using CL594-60229

    IF Staining of C2C12 using CL594-60229

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CL594-60229 (Myosin Light Chain 2 antibody) at dilution of 1:50. .

  • IF Staining of mouse heart using CL594-60229

    IF Staining of mouse heart using CL594-60229

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite®594 Myosin Light Chain 2 antibody (CL594-60229, Clone: 5A1F1 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy MYL2 Ready-To-Use IHC Kit. MYL2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60229-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60229 targets Myosin Light Chain 2 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Myosin Light Chain 2 fusion protein Ag1356 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myosin, light chain 2, regulatory, cardiac, slow
Synonyms MLC 2v, MYL2, MLC-2s/v, MLC-2, MLC2
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC015821
Gene Symbol Myosin Light Chain 2
Gene ID (NCBI) 4633
RRIDAB_2883447
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP10916
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.

实验方案

Product Specific Protocols
IF protocol for CL594 Myosin Light Chain 2 antibody CL594-60229Download protocol
Standard Protocols
Click here to view our Standard Protocols