验证数据展示
经过测试的应用
Positive IF/ICC detected in | C2C12 cells |
Planning an IHC experiment? We recommend our IHCeasy MYL2 Ready-To-Use IHC Kit. MYL2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60229-1-Ig
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-60229 targets Myosin Light Chain 2 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human, mouse, rat, pig |
免疫原 | Myosin Light Chain 2 fusion protein Ag1356 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | myosin, light chain 2, regulatory, cardiac, slow |
别名 | CMH10, DKFZp779C0562, MLC 2, MLC 2v, MLC2, MLC2v, MYL2, Myosin Light Chain 2, Myosin Light Chain 2/MLC-2V |
计算分子量 | 19 kDa |
观测分子量 | 18 kDa |
GenBank蛋白编号 | BC015821 |
基因名称 | Myosin Light Chain 2 |
Gene ID (NCBI) | 4633 |
RRID | AB_2883123 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P10916 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 Myosin Light Chain 2 antibody CL488-60229 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |