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验证数据展示

  • IF Staining of C2C12 using CL488-60229

    IF Staining of C2C12 using CL488-60229

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CoraLite® Plus 488 Myosin Light Chain 2 antibody (CL488-60229, Clone: 5A1F1 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Myosin Light Chain 2 Monoclonal antibody

Myosin Light Chain 2 Monoclonal Antibody for IF/ICC
Cat No. CL488-60229

Print datasheet

CloneNo. 5A1F1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC

Synonyms

CMH10, DKFZp779C0562, MLC 2, MLC 2v, MLC2, MLC2v, MYL2, Myosin Light Chain 2, Myosin Light Chain 2/MLC-2V

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy MYL2 Ready-To-Use IHC Kit. MYL2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60229-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60229 targets Myosin Light Chain 2 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Myosin Light Chain 2 fusion protein Ag1356 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myosin, light chain 2, regulatory, cardiac, slow
Synonyms CMH10, DKFZp779C0562, MLC 2, MLC 2v, MLC2, MLC2v, MYL2, Myosin Light Chain 2, Myosin Light Chain 2/MLC-2V
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC015821
Gene Symbol Myosin Light Chain 2
Gene ID (NCBI) 4633
RRIDAB_2883123
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP10916
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Myosin Light Chain 2 antibody CL488-60229Download protocol
Standard Protocols
Click here to view our Standard Protocols