Myosin Light Chain 2/MLC-2V Monoclonal antibody

Myosin Light Chain 2/MLC-2V Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60229-1-Ig

产品说明书

CloneNo. 5A1F1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, ELISA and More (1)

MLC 2v, MYL2, MLC-2s/v, MLC-2, MLC2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman heart tissue, pig heart tissue, rat heart tissue, rabbit heart tissue
Positive IHC detected inmouse heart tissue, human heart tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy MYL2 Ready-To-Use IHC Kit. MYL2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60229-1-Ig targets Myosin Light Chain 2/MLC-2V in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat, pig, rabbit
文献引用反应性human, rat
免疫原 Myosin Light Chain 2/MLC-2V fusion protein Ag1356 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 myosin, light chain 2, regulatory, cardiac, slow
别名 MLC 2v, MYL2, MLC-2s/v, MLC-2, MLC2
计算分子量 19 kDa
观测分子量 18 kDa
GenBank蛋白编号BC015821
基因名称 Myosin Light Chain 2
Gene ID (NCBI) 4633
RRIDAB_2881356
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP10916
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.

实验方案

Product Specific Protocols
WB protocol for Myosin Light Chain 2/MLC-2V antibody 60229-1-IgDownload protocol
IHC protocol for Myosin Light Chain 2/MLC-2V antibody 60229-1-IgDownload protocol
IF protocol for Myosin Light Chain 2/MLC-2V antibody 60229-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Stem Cells

Nitric Oxide-cGMP-PKG Pathway Acts on Orai1 to Inhibit the Hypertrophy of Human Embryonic Stem Cell-Derived Cardiomyocytes.

Authors - Y Wang
ratWB

Int J Stem Cells

Synergistic Effect of Hydrogen and 5-Aza on Myogenic Differentiation through the p38 MAPK Signaling Pathway in Adipose-Derived Mesenchymal Stem Cells

Authors - Jingcheng Wang
humanIF

Stem Cell Rev Rep

MYOCD is Required for Cardiomyocyte-like Cells Induction from Human Urine Cells and Fibroblasts Through Remodeling Chromatin.

Authors - Xiangyu Zhang
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