验证数据展示
经过测试的应用
Positive WB detected in | human heart tissue, pig heart tissue, rat heart tissue, rabbit heart tissue |
Positive IHC detected in | mouse heart tissue, human heart tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | C2C12 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IF | See 2 publications below |
产品信息
60229-1-Ig targets Myosin Light Chain 2/MLC-2V in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human, mouse, rat, pig, rabbit |
文献引用反应性 | human, rat |
免疫原 | Myosin Light Chain 2/MLC-2V fusion protein Ag1356 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | myosin, light chain 2, regulatory, cardiac, slow |
别名 | MLC 2v, MYL2, MLC-2s/v, MLC-2, MLC2 |
计算分子量 | 19 kDa |
观测分子量 | 18 kDa |
GenBank蛋白编号 | BC015821 |
基因名称 | Myosin Light Chain 2 |
Gene ID (NCBI) | 4633 |
RRID | AB_2881356 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P10916 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.
实验方案
Product Specific Protocols | |
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WB protocol for Myosin Light Chain 2/MLC-2V antibody 60229-1-Ig | Download protocol |
IHC protocol for Myosin Light Chain 2/MLC-2V antibody 60229-1-Ig | Download protocol |
IF protocol for Myosin Light Chain 2/MLC-2V antibody 60229-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Stem Cells Nitric Oxide-cGMP-PKG Pathway Acts on Orai1 to Inhibit the Hypertrophy of Human Embryonic Stem Cell-Derived Cardiomyocytes. | ||
Int J Stem Cells Synergistic Effect of Hydrogen and 5-Aza on Myogenic Differentiation through the p38 MAPK Signaling Pathway in Adipose-Derived Mesenchymal Stem Cells | ||
Stem Cell Rev Rep MYOCD is Required for Cardiomyocyte-like Cells Induction from Human Urine Cells and Fibroblasts Through Remodeling Chromatin. |