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Myosin Light Chain 2/MLC-2V Monoclonal antibody, PBS Only

Myosin Light Chain 2/MLC-2V Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5A1F1

Cat no : 60229-1-PBS

Print datasheet

Synonyms

CMH10, MLC 2, MLC 2v, MLC2, MLC2v, MYL2, Myosin Light Chain 2, Myosin Light Chain 2/MLC-2V

验证数据展示

  • IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)

    IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 0x lens). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYL2 antibody included
  • IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)

    IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 0x lens). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYL2 antibody included
  • IF Staining of C2C12 using 60229-1-Ig (same clone as 60229-1-PBS)

    IF Staining of C2C12 using 60229-1-Ig (same clone as 60229-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cell using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

  • WB analysis using 60229-1-Ig (same clone as 60229-1-PBS)

    WB analysis using 60229-1-Ig (same clone as 60229-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 60229-1-Ig (same clone as 60229-1-PBS)

    IHC staining of mouse skeletal muscle using 60229-1-Ig (same clone as 60229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYL2 antibody included
  • IHC staining of mouse heart using 60229-1-Ig (same clone as 60229-1-PBS)

    IHC staining of mouse heart using 60229-1-Ig (same clone as 60229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYL2 antibody included
  • IF Staining of C2C12 using 60229-1-Ig (same clone as 60229-1-PBS)

    IF Staining of C2C12 using 60229-1-Ig (same clone as 60229-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Myosin Light Chain 2/MLC-2V antibody (60229-1-Ig, Clone: 5A1F1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MYL2 Ready-To-Use IHC Kit. MYL2 primary antibody included.

产品信息

60229-1-PBS targets Myosin Light Chain 2/MLC-2V in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen Myosin Light Chain 2/MLC-2V fusion protein Ag1356 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myosin, light chain 2, regulatory, cardiac, slow
Synonyms CMH10, MLC 2, MLC 2v, MLC2, MLC2v, MYL2, Myosin Light Chain 2, Myosin Light Chain 2/MLC-2V
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC015821
Gene Symbol Myosin Light Chain 2
Gene ID (NCBI) 4633
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10916
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.