验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue, HepG2 cells, pig cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue, mouse brain tissue, rabbit brain tissue |
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66492-1-Ig targets NRG1, isoform SMDF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig, mouse, rat, rabbit samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | Human, pig, mouse, rat, rabbit |
| 文献引用反应性 | mouse, rat |
| 免疫原 | NRG1, isoform SMDF fusion protein Ag0803 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | neuregulin 1 |
| 别名 | ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, NRG1, Pro NRG1, SMDF |
| 计算分子量 | 70 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC007675 |
| 基因名称 | NRG1 |
| Gene ID (NCBI) | 3084 |
| RRID | AB_2881857 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q02297 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The antibody is specific to NRG1 isoform SMDF.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NRG1, isoform SMDF antibody 66492-1-Ig | Download protocol |
| IF protocol for NRG1, isoform SMDF antibody 66492-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Prog Neuropsychopharmacol Biol Psychiatry The Notch1/Hes1 pathway regulates Neuregulin 1/ErbB4 and participates in microglial activation in rats with VPA-induced autism | ||
Int J Mol Sci Dysregulation of ErbB4 Signaling Pathway in the Dorsal Hippocampus after Neonatal Hypoxia-Ischemia and Late Deficits in PV+ Interneurons, Synaptic Plasticity and Working Memory |
