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验证数据展示

  • IF Staining of MCF-7 using CL594-66492

    IF Staining of MCF-7 using CL594-66492

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CL594-66492 (NRG1, isoform SMDF antibody) at dilution of 1:100.

CoraLite®594-conjugated NRG1, isoform SMDF Monoclonal antibody

NRG1, isoform SMDF Monoclonal Antibody for IF/ICC
Cat No. CL594-66492

产品说明书

CloneNo. 2C6D12

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig, mouse, rat

应用

IF/ICC

别名

ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, NRG1, NRG1, isoform SMDF, Pro NRG1, SMDF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66492-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66492 targets NRG1, isoform SMDF in IF/ICC applications and shows reactivity with Human, pig, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, pig, mouse, rat
免疫原 NRG1, isoform SMDF fusion protein Ag0803 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 neuregulin 1
别名 ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, NRG1, NRG1, isoform SMDF, Pro NRG1, SMDF
计算分子量 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC007675
基因名称 NRG1
Gene ID (NCBI) 3084
RRIDAB_2883595
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ02297
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The antibody is specific to NRG1 isoform SMDF.

实验方案

Product Specific Protocols
IF protocol for CL594 NRG1, isoform SMDF antibody CL594-66492Download protocol
Standard Protocols
Click here to view our Standard Protocols