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CoraLite®594-conjugated NRG1, isoform SMDF Monoclonal antibody

NRG1, isoform SMDF Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2C6D12

Cat no : CL594-66492

Print datasheet

Synonyms

ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, NRG1, NRG1, isoform SMDF, Pro NRG1, SMDF

验证数据展示

  • IF Staining of MCF-7 using CL594-66492

    IF Staining of MCF-7 using CL594-66492

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CL594-66492 (NRG1, isoform SMDF antibody) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66492-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66492 targets NRG1, isoform SMDF in IF/ICC applications and shows reactivity with Human, pig, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, pig, mouse, rat
Immunogen NRG1, isoform SMDF fusion protein Ag0803 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name neuregulin 1
Synonyms ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, NRG1, NRG1, isoform SMDF, Pro NRG1, SMDF
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC007675
Gene Symbol NRG1
Gene ID (NCBI) 3084
RRIDAB_2883595
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ02297
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The antibody is specific to NRG1 isoform SMDF.

实验方案

Product Specific Protocols
IF protocol for CL594 NRG1, isoform SMDF antibody CL594-66492Download protocol
Standard Protocols
Click here to view our Standard Protocols