验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MCF-7 cells |
For other applications, we recommend the unconjugated version of this antibody, 66492-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-66492 targets NRG1, isoform SMDF in IF/ICC applications and shows reactivity with Human, pig, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Cited Applications | IF |
| Tested Reactivity | Human, pig, mouse, rat |
| Immunogen | NRG1, isoform SMDF fusion protein Ag0803 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | neuregulin 1 |
| Synonyms | ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, NRG1, NRG1, isoform SMDF, Pro NRG1, SMDF |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC007675 |
| Gene Symbol | NRG1 |
| Gene ID (NCBI) | 3084 |
| RRID | AB_2883347 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q02297 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The antibody is specific to NRG1 isoform SMDF.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NRG1, isoform SMDF antibody CL488-66492 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |