验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MCF-7 cells |
For other applications, we recommend the unconjugated version of this antibody, 66492-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-66492 targets NRG1, isoform SMDF in IF/ICC applications and shows reactivity with Human, pig, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | Human, pig, mouse, rat |
| 免疫原 | NRG1, isoform SMDF fusion protein Ag0803 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | neuregulin 1 |
| 别名 | ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, NRG1, NRG1, isoform SMDF, Pro NRG1, SMDF |
| 计算分子量 | 70 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC007675 |
| 基因名称 | NRG1 |
| Gene ID (NCBI) | 3084 |
| RRID | AB_2883347 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q02297 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The antibody is specific to NRG1 isoform SMDF.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NRG1, isoform SMDF antibody CL488-66492 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |