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PARK7/DJ-1 Monoclonal antibody

PARK7/DJ-1 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

4G4E7

Cat no : 68915-6-Ig

Print datasheet

Synonyms

PARK7, PARK7,DJ-1, Maillard deglycase, EC:3.1.2.-, EC:3.5.1.-, EC:3.5.1.124, DJ-1

验证数据展示

  • WB analysis using 68915-6-Ig

    WB analysis using 68915-6-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68915-6-Ig (PARK7/DJ-1 antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours.

  • WB analysis using 68915-6-Ig

    WB analysis using 68915-6-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68915-6-Ig (PARK7/DJ-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of SKOV-3 using 68915-6-Ig

    FC experiment of SKOV-3 using 68915-6-Ig

    1x10^6 SKOV-3 cells were intracellularly stained with 0.2 μg PARK7/DJ-1 Monoclonal antibody (68915-6-Ig, Clone: 4G4E7, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA.

  • FC experiment of HeLa using 68915-6-Ig

    FC experiment of HeLa using 68915-6-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.2 μg PARK7/DJ-1 Monoclonal antibody (68915-6-Ig, Clone: 4G4E7, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA.

  • FC experiment of Jurkat using 68915-6-Ig

    FC experiment of Jurkat using 68915-6-Ig

    1x10^6 Jurkat cells were intracellularly stained with 0.2 μg PARK7/DJ-1 Monoclonal antibody (68915-6-Ig, Clone: 4G4E7, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA.

  • FC experiment of Raji using 68915-6-Ig

    FC experiment of Raji using 68915-6-Ig

    1x10^6 Raji cells were intracellularly stained with 0.2 μg PARK7/DJ-1 Monoclonal antibody (68915-6-Ig, Clone: 4G4E7, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA.

经过测试的应用

Positive WB detected inHeLa cells, pig brain tissue, HEK-293 cells, Jurkat cells, PC-12 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive FC (Intra) detected inHeLa cells, Raji cells, Jurkat cells, SKOV-3 cells
Planning an IHC experiment? We recommend our IHCeasy PARK7 Ready-To-Use IHC Kit. PARK7 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:19400
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68915-6-Ig targets PARK7/DJ-1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen PARK7/DJ-1 fusion protein Ag28526 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Parkinson disease (autosomal recessive, early onset) 7
Synonyms PARK7, PARK7,DJ-1, Maillard deglycase, EC:3.1.2.-, EC:3.5.1.-, EC:3.5.1.124, DJ-1
Calculated Molecular Weight 189 aa, 20 kDa
Observed Molecular Weight20-25 kDa
GenBank Accession NumberBC008188
Gene Symbol PARK7
Gene ID (NCBI) 11315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99497
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).

实验方案

Product Specific Protocols
WB protocol for PARK7/DJ-1 antibody 68915-6-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols