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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68915-6-Ig (same clone as 68915-6-PBS)

    WB analysis using 68915-6-Ig (same clone as 68915-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68915-6-Ig (PARK7/DJ-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

  • WB analysis using 68915-6-Ig (same clone as 68915-6-PBS)

    WB analysis using 68915-6-Ig (same clone as 68915-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68915-6-Ig (PARK7/DJ-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

  • FC experiment of SKOV-3 using 68915-6-Ig (same clone as 68915-6-PBS)

    FC experiment of SKOV-3 using 68915-6-Ig (same clone as 68915-6-PBS)

    1x10^6 SKOV-3 cells were intracellularly stained with 0.2 μg PARK7/DJ-1 Monoclonal antibody (68915-6-Ig, Clone: 4G4E7, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68915-6-Ig (same clone as 68915-6-PBS)

    FC experiment of HeLa using 68915-6-Ig (same clone as 68915-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.2 μg PARK7/DJ-1 Monoclonal antibody (68915-6-Ig, Clone: 4G4E7, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 68915-6-Ig (same clone as 68915-6-PBS)

    FC experiment of Jurkat using 68915-6-Ig (same clone as 68915-6-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.2 μg PARK7/DJ-1 Monoclonal antibody (68915-6-Ig, Clone: 4G4E7, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

  • FC experiment of Raji using 68915-6-Ig (same clone as 68915-6-PBS)

    FC experiment of Raji using 68915-6-Ig (same clone as 68915-6-PBS)

    1x10^6 Raji cells were intracellularly stained with 0.2 μg PARK7/DJ-1 Monoclonal antibody (68915-6-Ig, Clone: 4G4E7, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

PARK7/DJ-1 Monoclonal antibody, PBS Only

PARK7/DJ-1 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 68915-6-PBS

产品说明书

CloneNo. 4G4E7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, FC (Intra), Indirect ELISA

别名

PARK7, PARK7,DJ-1, 4G4E7, DJ 1, DJ1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PARK7 Ready-To-Use IHC Kit. PARK7 primary antibody included.

产品信息

68915-6-PBS targets PARK7/DJ-1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 PARK7/DJ-1 fusion protein Ag28526 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Parkinson disease (autosomal recessive, early onset) 7
别名 PARK7, PARK7,DJ-1, 4G4E7, DJ 1, DJ1
计算分子量 189 aa, 20 kDa
观测分子量20-25 kDa
GenBank蛋白编号BC008188
基因名称 PARK7
Gene ID (NCBI) 11315
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99497
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).