验证数据展示
经过测试的应用
| Positive WB detected in | human plasma tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IHC | See 2 publications below |
产品信息
66399-1-Ig targets Plasminogen in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | Plasminogen fusion protein Ag9938 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | plasminogen |
| 别名 | PLG, Plasmin heavy chain A, short form, EC:3.4.21.7, Angiostatin, 1F6A11 |
| 计算分子量 | 810 aa, 91 kDa |
| 观测分子量 | 105 kDa |
| GenBank蛋白编号 | BC060513 |
| 基因名称 | Plasminogen |
| Gene ID (NCBI) | 5340 |
| RRID | AB_2881773 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P00747 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Plasminogen is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasminogen plays an important role in fibrinolysis as well as wound healing, cell migration, tissue modeling and angiogenesis. Plasminogen is synthesized in the liver, and it circulates in the blood, with a half-life of 2.2 days. Plasminogen is the precursor of plasmin, which lyses fibrin clots to fibrin degradation products (FDP) and D-dimer; the conversion to active protease is mediated by tissue-type (tPA) and urokinase-type (uPA) plasminogen activators. Defects in plasminogen are likely a cause of thrombophilia and ligneous conjunctivitis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Plasminogen antibody 66399-1-Ig | Download protocol |
| IHC protocol for Plasminogen antibody 66399-1-Ig | Download protocol |
| IF protocol for Plasminogen antibody 66399-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium. | ||
Front Cell Dev Biol Elevated Levels of miR-144-3p Induce Cholinergic Degeneration by Impairing the Maturation of NGF in Alzheimer's Disease. | ||
Biomed Pharmacother Proteomic and mechanistic study of Qingxuan Tongluo formula and curcumin in the treatment of Mycoplasma pneumoniae pneumonia. | ||
Clin Transl Oncol A prognostic gene signature for gastric cancer and the immune infiltration-associated mechanism underlying the signature gene, PLG |