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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PRDX3 Recombinant antibody

PRDX3 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1L11

Cat no : CL488-81833

Print datasheet

Synonyms

MER5, EC:1.11.1.24, AOP-1, AOP1, Antioxidant protein 1

验证数据展示

  • IF Staining of HepG2 using CL488-81833

    IF Staining of HepG2 using CL488-81833

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PRDX3 antibody (CL488-81833, Clone: 1L11 ) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-81833

    FC experiment of MCF-7 using CL488-81833

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PRDX3 (CL488-81833, Clone:1L11)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PRDX3 Ready-To-Use IHC Kit. PRDX3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81833-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-81833 targets PRDX3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen PRDX3 fusion protein Ag1062 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name peroxiredoxin 3
Synonyms MER5, EC:1.11.1.24, AOP-1, AOP1, Antioxidant protein 1
Calculated Molecular Weight 27 kDa
Observed Molecular Weight28 kDa
GenBank Accession NumberBC007062
Gene Symbol PRDX3
Gene ID (NCBI) 10935
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30048
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells (PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PRDX3 antibody CL488-81833Download protocol
Standard Protocols
Click here to view our Standard Protocols