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验证数据展示

  • IF Staining of HeLa using CL488-82960-5

    IF Staining of HeLa using CL488-82960-5

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 RAB32 antibody (CL488-82960-5, Clone: 230315B10 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-82960-5

    FC experiment of HeLa using CL488-82960-5

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Rab32 Recombinant Antibody (CL488-82960-5, Clone:230315B10)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated RAB32 Recombinant antibody

RAB32 Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-82960-5

产品说明书

CloneNo. 230315B10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

Ras-related protein Rab-32, Ras related protein Rab 32, 230315B10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells, U-251 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAB32 Ready-To-Use IHC Kit. RAB32 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82960-5-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82960-5 targets RAB32 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 RAB32 fusion protein Ag1450 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB32, member RAS oncogene family
别名 Ras-related protein Rab-32, Ras related protein Rab 32, 230315B10
计算分子量 25 kDa
GenBank蛋白编号BC015061
基因名称 RAB32
Gene ID (NCBI) 10981
RRIDAB_3673153
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13637
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab32 belongs to the Ras-like small GTPase superfamily, which regulate membrane dynamics, including membrane fusion/fission, exocytosis, and cytoskeletal trafficking. Rab32 anchors the type II regulatory subunit of protein kinase A to the mitochondrion and aids in mitochondrial fission. TRab32 also appears to be involved in autophagy and melanosome secretion.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RAB32 antibody CL488-82960-5Download protocol
Standard Protocols
Click here to view our Standard Protocols