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CoraLite® Plus 488-conjugated RAB32 Recombinant antibody

RAB32 Uni-rAbTM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230315B10

Cat no : CL488-82960-5

Print datasheet

Synonyms

Ras-related protein Rab-32, Ras related protein Rab 32, 230315B10

验证数据展示

  • IF Staining of HeLa using CL488-82960-5

    IF Staining of HeLa using CL488-82960-5

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 RAB32 antibody (CL488-82960-5, Clone: 230315B10 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-82960-5

    FC experiment of HeLa using CL488-82960-5

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Rab32 Recombinant Antibody (CL488-82960-5, Clone:230315B10)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAB32 Ready-To-Use IHC Kit. RAB32 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82960-5-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-82960-5 is RAB32 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen RAB32 fusion protein Ag1450 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RAB32, member RAS oncogene family
Synonyms Ras-related protein Rab-32, Ras related protein Rab 32, 230315B10
Calculated Molecular Weight 25 kDa
GenBank Accession NumberBC015061
Gene Symbol RAB32
Gene ID (NCBI) 10981
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13637
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab32 belongs to the Ras-like small GTPase superfamily, which regulate membrane dynamics, including membrane fusion/fission, exocytosis, and cytoskeletal trafficking. Rab32 anchors the type II regulatory subunit of protein kinase A to the mitochondrion and aids in mitochondrial fission. TRab32 also appears to be involved in autophagy and melanosome secretion.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RAB32 antibody CL488-82960-5Download protocol
Standard Protocols
Click here to view our Standard Protocols