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验证数据展示

  • IF Staining of A431 using CL488-84330

    IF Staining of A431 using CL488-84330

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 RBBP7 antibody (CL488-84330, Clone: 241189D6 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-84330

    FC experiment of HeLa using CL488-84330

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug Coralite® Plus 488 Rbbp7 Recombinant Antibody (CL488-84330, Clone:241189D6)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated RbAp46 Recombinant antibody

RbAp46 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-84330

产品说明书

CloneNo. 241189D6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

RBBP7, Retinoblastoma-binding protein 7, RBBP-7, Nucleosome-remodeling factor subunit RBAP46, Histone-binding protein RBBP7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RBBP7 Ready-To-Use IHC Kit. RBBP7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84330-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84330 targets RbAp46 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 retinoblastoma binding protein 7
别名 RBBP7, Retinoblastoma-binding protein 7, RBBP-7, Nucleosome-remodeling factor subunit RBAP46, Histone-binding protein RBBP7
计算分子量 48 kDa
GenBank蛋白编号NM_002893
基因名称 RBBP7
Gene ID (NCBI) 5931
RRIDAB_3673354
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16576
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBBP7, also named as RBAP46, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP7 is a core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP7.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RbAp46 antibody CL488-84330Download protocol
Standard Protocols
Click here to view our Standard Protocols