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验证数据展示

  • WB analysis of HepG2 using 84496-1-RR (same clone as 84496-1-PBS)

    WB analysis of HepG2 using 84496-1-RR (same clone as 84496-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 84496-1-RR (RBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84496-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01338-1

    Sandwich ELISA standard curve of MP01338-1

    Sandwich ELISA standard curve of MP01338-1, Human RBP4 Recombinant Matched Antibody Pair - PBS only. 84496-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg31642. 84496-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

  • Sample test of MP01338-1

    Sample test of MP01338-1

    Urine of six individual healthy human donors was measured. The RBP4 concentration of detected samples was determined to be 90.68 ng/mL with a range of 29.96 - 290.83 ng/mL.

  • Sample test of MP01338-1

    Sample test of MP01338-1

    Saliva of six individual healthy human donors was measured. The RBP4 concentration of detected samples was determined to be 12.14 ng/mL with a range of 8.34-17.17 ng/mL.

  • Sample test of MP01338-1

    Sample test of MP01338-1

    HepG2 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human RBP4, and measured 707.54 ng/mL.

  • Sample test of MP01338-1

    Sample test of MP01338-1

    Serum of twelve individual healthy human donors was measured. The RBP4 concentration of detected samples was determined to be 87,804.85 ng/mL with a range of 37,160.99 - 156,178.64 ng/mL.

  • FC experiment of HepG2 using 84496-1-RR (same clone as 84496-1-PBS)

    FC experiment of HepG2 using 84496-1-RR (same clone as 84496-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RBP4 Recombinant antibody (84496-1-RR, Clone:241944B3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84496-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01338-1

    Cytometric bead array standard curve of MP01338-1

    Cytometric bead array standard curve of MP01338-1, RBP4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84496-1-PBS. Detection antibody: 84496-2-PBS. Standard: Eg31642. Range: 0.156-20 ng/mL.

  • Cytometric bead array standard curve of MP01338-2

    Cytometric bead array standard curve of MP01338-2

    Cytometric bead array standard curve of MP01338-2, RBP4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84496-1-PBS. Detection antibody: 84496-4-PBS. Standard: Eg31642. Range: 0.156-20 ng/mL.

  • Cytometric bead array standard curve of MP01338-3

    Cytometric bead array standard curve of MP01338-3

    Cytometric bead array standard curve of MP01338-3, RBP4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84496-1-PBS. Detection antibody: 84496-3-PBS. Standard: Eg31642. Range: 0.313-20 ng/mL.

  • Affinity and Kinetic Characterization of 84496-1-RR

    Affinity and Kinetic Characterization of 84496-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84496-1-RR against Human RBP4 were performed. The affinity constant is 0.504 nM.

RBP4 Recombinant antibody, PBS Only (Capture)

RBP4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84496-1-PBS

产品说明书

CloneNo. 241944B3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176), Plasma retinol-binding protein, Plasma retinol binding protein

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RBP4 Ready-To-Use IHC Kit. RBP4 primary antibody included.

产品信息

84496-1-PBS targets RBP4 as part of a matched antibody pair:

MP01338-1: 84496-1-PBS capture and 84496-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01338-2: 84496-1-PBS capture and 84496-4-PBS detection (validated in Cytometric bead array)

MP01338-3: 84496-1-PBS capture and 84496-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 retinol binding protein 4, plasma
别名 Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176), Plasma retinol-binding protein, Plasma retinol binding protein
计算分子量 201 aa, 23 kDa
观测分子量20-21 kDa
GenBank蛋白编号BC020633
基因名称 RBP4
Gene ID (NCBI) 5950
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02753
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)