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ChromoTek RFP-Trap® Magnetic Particles M-270

RFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Magnetic Particles M-270.

Specificity

mCherry, mRFP, mPlum

Applications

IP, CoIP

Conjugate

Magnetic Particles M-270

Cat No : rtd

Synonyms

Magnetic Particles M-270, RFP Magnetic Particles M-270, RFP tag, RFP-tag, RFP-Trap® , RFP-Trap® Magnetic Particles M-270, rtd, rtd-10, rtd-100, rtd-20, rtd-200, rtd-400

Validation Data Gallery

  • Immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound

    Immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound

  • Coomassie and Western blot to show the effectivity of immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound

    Coomassie and Western blot to show the effectivity of immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound

  • Cartoon to visualize the binding of large GFP-fusion proteins (GFP (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (GFP (green) + protein of interest (POI, blue) to the GFP VHH (dark green) of GFP-Trap Magnetic Agarose or GFP-Trap Magnetic Particles M-270.

    Cartoon to visualize the binding of large GFP-fusion proteins (GFP (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (GFP (green) + protein of interest (POI, blue) to the GFP VHH (dark green) of GFP-Trap Magnetic Agarose or GFP-Trap Magnetic Particles M-270.

Product Information

RFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Magnetic Particles M-270.

ApplicationsIP, CoIP
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 5 nM
Specificity/TargetmRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
GeneID (NCBI)0
Coupled VHHRecombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment
ConjugateMagnetic Particles M-270
Beads~ 2.8 µm (Magnetic, high throughput-compatible)
Binding capacity10 µL slurry bind ~ 0.5 µg of recombinant mCherry
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer1x PBS, Preservative: 0.09 % sodium azide
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Publications

ApplicationTitle
IP

Cell Rep

RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site.

Authors - Jasmine George
View Article