ChromoTek RFP-Trap® Magnetic Particles M-270
RFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Magnetic Particles M-270.
Cat No : rtd
Synonyms
Validation Data Gallery
Product Information
RFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Magnetic Particles M-270.
Applications | IP, CoIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 5 nM |
Specificity/Target | mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet |
GeneID (NCBI) | 0 |
Coupled VHH | Recombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment |
Conjugate | Magnetic Particles M-270 |
Beads | ~ 2.8 µm (Magnetic, high throughput-compatible) |
Binding capacity | 10 µL slurry bind ~ 0.5 µg of recombinant mCherry |
Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
Storage Buffer | 1x PBS, Preservative: 0.09 % sodium azide |
Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
SDS |
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SDS RFP-Trap Magnetic Particles M-270 |
RFP-Trap Specificity |
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Fluorescent protein specificity table |
PROTOCOL |
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PROTOCOL RFP-Trap Magnetic Particles M-270 |
Trouble shooting |
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Troubleshooting guide immunoprecipitation (IP) |
Brochure |
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RFP-Trap product brochure |
Publications
Application | Title |
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Cell Rep RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site. |