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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82271-8-RR (same clone as 82271-8-PBS)

    WB analysis using 82271-8-RR (same clone as 82271-8-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82271-8-RR (S100B antibody) at dilution of 1:5150 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82271-8-PBS in a different storage buffer formulation.

  • IHC staining of Human appendicitis using 82271-8-RR (same clone as 82271-8-PBS)

    IHC staining of Human appendicitis using 82271-8-RR (same clone as 82271-8-PBS)

    Immunohistochemical analysis of paraffin-embedded Human appendicitis tissue slide using 82271-8-RR (S100B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82271-8-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100B antibody included
  • IHC staining of Human appendicitis using 82271-8-RR (same clone as 82271-8-PBS)

    IHC staining of Human appendicitis using 82271-8-RR (same clone as 82271-8-PBS)

    Immunohistochemical analysis of paraffin-embedded Human appendicitis tissue slide using 82271-8-RR (S100B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82271-8-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100B antibody included
  • FC experiment of A375 using 82271-8-RR (same clone as 82271-8-PBS)

    FC experiment of A375 using 82271-8-RR (same clone as 82271-8-PBS)

    1x10^6 A375 cells were intracellularly stained with 0.25 ug S100B Recombinant antibody (82271-8-RR, Clone:1E2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82271-8-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82271-8-RR

    Affinity and Kinetic Characterization of 82271-8-RR

    Biolayer interferometry (BLl) kinetic assays of 82271-8-RR against Human S100 Beta were performed. The affinity constant is 38.5 pM.

S100B Recombinant antibody, PBS Only

S100B Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 82271-8-PBS

产品说明书

CloneNo. 1E2

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

S100 beta, S-100 protein beta chain, S100 calcium-binding protein B, Protein S100-B, Protein S100 B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy S100B Ready-To-Use IHC Kit. S100B primary antibody included.

产品信息

82271-8-PBS targets S100B in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 S100B fusion protein Ag7440 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 S100 calcium binding protein B
别名 S100 beta, S-100 protein beta chain, S100 calcium-binding protein B, Protein S100-B, Protein S100 B
计算分子量 11 kDa
观测分子量11 kDa
GenBank蛋白编号BC001766
基因名称 S100 Beta
Gene ID (NCBI) 6285
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04271
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. In the nervous system, S100B is constitutively released by astrocytes into the extracellular space and at nanomolar concentrations, it can promote neurite outgrowth and protect neurons against oxidative stress. Within the central nervous system (CNS), S100B is thought to be a marker for astroglial activation, linking astrocyte dysfunction to schizophrenia. In addition to astrocytes, S100B is released from many cell types, such as, adipocytes, chondrocytes, cardiomyocytes and lymphocytes. Serum S100B represents a new biomarker for mood disorders.