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验证数据展示

  • WB analysis using 82271-8-RR

    WB analysis using 82271-8-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82271-8-RR (S100B antibody) at dilution of 1:5150 incubated at room temperature for 1.5 hours.

  • IHC staining of Human appendicitis using 82271-8-RR

    IHC staining of Human appendicitis using 82271-8-RR

    Immunohistochemical analysis of paraffin-embedded Human appendicitis tissue slide using 82271-8-RR (S100B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100B antibody included
  • IHC staining of Human appendicitis using 82271-8-RR

    IHC staining of Human appendicitis using 82271-8-RR

    Immunohistochemical analysis of paraffin-embedded Human appendicitis tissue slide using 82271-8-RR (S100B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100B antibody included
  • FC experiment of A375 using 82271-8-RR

    FC experiment of A375 using 82271-8-RR

    1x10^6 A375 cells were intracellularly stained with 0.25 ug S100B Recombinant antibody (82271-8-RR, Clone:1E2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82271-8-RR

    Affinity and Kinetic Characterization of 82271-8-RR

    Biolayer interferometry (BLl) kinetic assays of 82271-8-RR against Human S100 Beta were performed. The affinity constant is 38.5 pM.

S100B Recombinant antibody

S100B Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 82271-8-RR

Print datasheet

CloneNo. 1E2

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

S100 beta, S-100 protein beta chain, S100 calcium-binding protein B, Protein S100-B, Protein S100 B

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inC6 cells, A375 cells, rat brain tissue
Positive IHC detected inHuman appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA375 cells
Planning an IHC experiment? We recommend our IHCeasy S100B Ready-To-Use IHC Kit. S100B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10300
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82271-8-RR targets S100B in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, rat
Immunogen S100B fusion protein Ag7440 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name S100 calcium binding protein B
Synonyms S100 beta, S-100 protein beta chain, S100 calcium-binding protein B, Protein S100-B, Protein S100 B
Calculated Molecular Weight 11 kDa
Observed Molecular Weight11 kDa
GenBank Accession NumberBC001766
Gene Symbol S100 Beta
Gene ID (NCBI) 6285
RRIDAB_3670520
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04271
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. In the nervous system, S100B is constitutively released by astrocytes into the extracellular space and at nanomolar concentrations, it can promote neurite outgrowth and protect neurons against oxidative stress. Within the central nervous system (CNS), S100B is thought to be a marker for astroglial activation, linking astrocyte dysfunction to schizophrenia. In addition to astrocytes, S100B is released from many cell types, such as, adipocytes, chondrocytes, cardiomyocytes and lymphocytes. Serum S100B represents a new biomarker for mood disorders.

实验方案

Product Specific Protocols
WB protocol for S100B antibody 82271-8-RRDownload protocol
IHC protocol for S100B antibody 82271-8-RRDownload protocol
FC protocol for S100B antibody 82271-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols