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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SH3BGR Polyclonal antibody

SH3BGR Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21161

Print datasheet

Synonyms

SH3BGR protein, SH3 domain-binding glutamic acid-rich protein, SH3 domain binding glutamic acid rich protein, 21-glutamic acid-rich protein

验证数据展示

  • IF Staining of HeLa using CL488-21161

    IF Staining of HeLa using CL488-21161

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 SH3BGR antibody (CL488-21161) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-21161

    FC experiment of HeLa using CL488-21161

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated SH3BGR Polyclonal antibody (CL488-21161)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 21161-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21161 targets SH3BGR in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen SH3BGR fusion protein Ag14739 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SH3 domain binding glutamic acid-rich protein
Synonyms SH3BGR protein, SH3 domain-binding glutamic acid-rich protein, SH3 domain binding glutamic acid rich protein, 21-glutamic acid-rich protein
Calculated Molecular Weight 239 aa, 26 kDa
Observed Molecular Weight 26-30 kDa
GenBank Accession NumberBC006371
Gene Symbol SH3BGR
Gene ID (NCBI) 6450
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55822
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SH3BGR antibody CL488-21161Download protocol
FC protocol for CL Plus 488 SH3BGR antibody CL488-21161Download protocol
Standard Protocols
Click here to view our Standard Protocols