验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | U2OS cells |
For other applications, we recommend the unconjugated version of this antibody, 82875-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82875 targets SHPK in FC (Intra) applications and shows reactivity with Human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | Human |
| 免疫原 | SHPK fusion protein Ag33893 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | sedoheptulokinase |
| 别名 | SHK, Sedoheptulokinase, EC:2.7.1.14, CARKL, Carbohydrate kinase-like protein |
| 计算分子量 | 478 aa, 52 kDa |
| GenBank蛋白编号 | BC020543 |
| 基因名称 | SHPK |
| Gene ID (NCBI) | 23729 |
| RRID | AB_3673091 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UHJ6 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SHPK, also named CARKL, is a carbohydrate kinase involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane (PubMed: 10673275). SHPK is a sedoheptulose kinase of the pentose phosphate pathway. It acts as a modulator of macrophage activation through control of glucose metabolism (PMID: 22682222).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 SHPK antibody CL488-82875 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |