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CoraLite® Plus 488-conjugated SHPK Recombinant antibody

SHPK Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230115A4

Cat no : CL488-82875

Print datasheet

Synonyms

SHK, Sedoheptulokinase, EC:2.7.1.14, CARKL, Carbohydrate kinase-like protein

验证数据展示

  • FC experiment of U2OS using CL488-82875

    FC experiment of U2OS using CL488-82875

    1x10^6 U2OS cells were intracellularly stained with 0.8 ug Coralite® Plus 488 SHPK Recombinant Antibody (CL488-82875, Clone:230115A4)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 82875-1-PBS

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82875 targets SHPK in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human
Immunogen SHPK fusion protein Ag33893 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sedoheptulokinase
Synonyms SHK, Sedoheptulokinase, EC:2.7.1.14, CARKL, Carbohydrate kinase-like protein
Calculated Molecular Weight 478 aa, 52 kDa
GenBank Accession NumberBC020543
Gene Symbol SHPK
Gene ID (NCBI) 23729
RRIDAB_3673091
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UHJ6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SHPK, also named CARKL, is a carbohydrate kinase involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane (PubMed: 10673275). SHPK is a sedoheptulose kinase of the pentose phosphate pathway. It acts as a modulator of macrophage activation through control of glucose metabolism (PMID: 22682222).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SHPK antibody CL488-82875Download protocol
Standard Protocols
Click here to view our Standard Protocols