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SLC35B4 Recombinant antibody, PBS Only (Capture)

SLC35B4 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240332F10

Cat no : 83527-3-PBS

Print datasheet

Synonyms

YEA4, YEA, UDP-xylose and UDP-N-acetylglucosamine transporter, PSEC0055, Nucleotide sugar transporter SLC35B4

验证数据展示

  • WB analysis of DU 145 using 83527-3-RR (same clone as 83527-3-PBS)

    WB analysis of DU 145 using 83527-3-RR (same clone as 83527-3-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 83527-3-RR (SLC35B4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83527-3-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83527-3-RR (same clone as 83527-3-PBS)

    FC experiment of HepG2 using 83527-3-RR (same clone as 83527-3-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Slc35b4 Recombinant Antibody (83527-3-RR, Clone:240332F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83527-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00497-1

    Cytometric bead array standard curve of MP00497-1

    Cytometric bead array standard curve of MP00497-1, SLC35B4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83527-3-PBS. Detection antibody: 83527-2-PBS. Standard: Ag22669. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83527-3-RR

    Affinity and Kinetic Characterization of 83527-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83527-3-RR against Human SLC35B4 were performed. The affinity constant is below 1 pM.

产品信息

83527-3-PBS targets SLC35B4 as part of a matched antibody pair:

MP00497-1: 83527-3-PBS capture and 83527-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen SLC35B4 fusion protein Ag22669 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name solute carrier family 35, member B4
Synonyms YEA4, YEA, UDP-xylose and UDP-N-acetylglucosamine transporter, PSEC0055, Nucleotide sugar transporter SLC35B4
GenBank Accession NumberBC008413
Gene Symbol SLC35B4
Gene ID (NCBI) 84912
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ969S0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C