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- KD/KO Validated
STAT3 Monoclonal antibody
STAT3 Monoclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, rat and More (1)
Applications
FC, IF/ICC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
3G2D12
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells, Jurkat cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human cervical cancer tissue, human heart tissue, human breast cancer tissue, human endometrial cancer tissue, human liver tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60199-1-Ig targets STAT3 in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, rat samples.
Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF, IHC, CoIP |
Tested Reactivity | human, rat |
Cited Reactivity | human, rat, zebrafish |
Immunogen | STAT3 fusion protein Ag0360 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | signal transducer and activator of transcription 3 (acute-phase response factor) |
Synonyms | Acute phase response factor, APRF, HIES, STAT3 |
Calculated Molecular Weight | 770 aa, 88 kDa |
Observed Molecular Weight | 70-88 kDa |
GenBank Accession Number | BC000627 |
Gene Symbol | STAT3 |
Gene ID (NCBI) | 6774 |
RRID | AB_10913811 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P40763 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for STAT3 antibody 60199-1-Ig | Download protocol |
IHC protocol for STAT3 antibody 60199-1-Ig | Download protocol |
IF protocol for STAT3 antibody 60199-1-Ig | Download protocol |
IP protocol for STAT3 antibody 60199-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
ACS Nano Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy. | ||
J Immunother Cancer Switch receptor T3/28 improves long-term persistence and antitumor efficacy of CAR-T cells | ||
Theranostics Combination therapy with ropivacaine-loaded liposomes and nutrient deprivation for simultaneous cancer therapy and cancer pain relief. | ||
Clin Transl Med PRKAR2A-derived circular RNAs promote the malignant transformation of colitis and distinguish patients with colitis-associated colorectal cancer. | ||
J Invest Dermatol Keratinocyte growth factor-2 regulates signal-transducing adaptor protein-2-mediated signal transducer and activator of transcription 3 signaling and reduces skin scar formation. | ||
Cell Biol Toxicol Protection of pancreatic β-cell by phosphocreatine through mitochondrial improvement via the regulation of dual AKT/IRS-1/GSK-3β and STAT3/Cyp-D signaling pathways |