验证数据展示

WB analysis using 18986-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 18986-1-AP
WB result of EGFR-Specific antibody (18986-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EGFR-Specific transfected HepG2 cells.
WB analysis of HeLa/RPE1 cells using 18986-1-AP
WB result ofanti-EGFR (18986-1-AP) in Hela and REP1 cell by Dr.Kodani, Andrew.
IP experiment of MCF-7 using 18986-1-AP
IP result of anti-EGFR-Specific (IP:18986-1-AP, 3ug; Detection:18986-1-AP 1:500) with MCF-7 cells lysate 2500ug.

Featured Product
KD/KO验证

EGFR-Specific Polyclonal antibody

EGFR-Specific Polyclonal Antibody for WB, IP, ELISA

Cat No. 18986-1-AP

产品说明书

引用文献(158)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (4)

应用

WB, IP, ELISA and More (4)

别名

EGFR, EGFR-Specific, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (158)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HepG2 cells, MCF-7 cells, A431 cells, HeLa/RPE1 cells, NCI-H1299 cells, HeLa cells, A549 cells, L02 cells, NCL-H1299 cells
Positive IP detected in	MCF-7 cells

Planning an IHC experiment? We recommend our IHCeasy EGFR Ready-To-Use IHC Kit. EGFR primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 7 publications below
WB	See 133 publications below
IHC	See 22 publications below
IF	See 20 publications below
IP	See 9 publications below
FC	See 1 publications below
CoIP	See 6 publications below
ChIP	See 1 publications below

产品信息

18986-1-AP targets EGFR-Specific in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

经测试应用	WB, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, ChIP
经测试反应性	human
文献引用反应性	human, mouse, rat, pig, sheep
免疫原	Peptide 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
别名	EGFR, EGFR-Specific, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1
计算分子量	134 kDa
观测分子量	145-165 kDa
GenBank蛋白编号	NM_005228
基因名称	EGFR
Gene ID (NCBI)	1956
RRID	AB_10596476
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P00533
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

ERBB1, also named as EGFR, is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer. The antibody is specific to isoform1.

实验方案

Product Specific Protocols
WB protocol for EGFR-Specific antibody 18986-1-AP	Download protocol
IP protocol for EGFR-Specific antibody 18986-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
		Nat Microbiol Interferon-induced transmembrane protein-1 competitively blocks Ephrin receptor A2-mediated Epstein-Barr virus entry into epithelial cells Authors - Yinggui Yang View Article
mouse	WB,IHC	Cell Res In vivo self-assembled small RNAs as a new generation of RNAi therapeutics. Authors - Zheng Fu KD Validated View Article
human	WB	Cancer Commun (Lond) Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma Authors - Xiaoteng Cui View Article
human	IF	ACS Appl Mater Interfaces On-Chip Replication of Extremely Early-Stage Tumor Behavior. Authors - Chengpan Li View Article
pig	WB	Biosens Bioelectron Microneedle patches integrated with lateral flow cassettes for blood-free chronic kidney disease point-of-care testing during a pandemic. Authors - Yi-Jyun Chen View Article
human	WB, IHC, ChIP	Cancer Res Hypoxia Alters the Response to Anti-EGFR Therapy by Regulating EGFR Expression and Downstream Signaling in a DNA Methylation-Specific and HIF-Dependent Manner. Authors - Mahelet Mamo View Article

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验证数据展示

WB analysis using 18986-1-AP

WB analysis of HepG2 using 18986-1-AP

WB analysis of HeLa/RPE1 cells using 18986-1-AP

IP experiment of MCF-7 using 18986-1-AP

EGFR-Specific Polyclonal antibody

EGFR-Specific Polyclonal Antibody for WB, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Interferon-induced transmembrane protein-1 competitively blocks Ephrin receptor A2-mediated Epstein-Barr virus entry into epithelial cells

In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.

Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma

On-Chip Replication of Extremely Early-Stage Tumor Behavior.

Microneedle patches integrated with lateral flow cassettes for blood-free chronic kidney disease point-of-care testing during a pandemic.

Hypoxia Alters the Response to Anti-EGFR Therapy by Regulating EGFR Expression and Downstream Signaling in a DNA Methylation-Specific and HIF-Dependent Manner.