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验证数据展示

  • WB analysis of Jurkat using 10508-1-AP

    WB analysis of Jurkat using 10508-1-AP

    WB result of Survivin antibody (10508-1-AP, 1:5000) with si-control and si-Survivin transfected Jurkat cells..

  • WB analysis using 10508-1-AP

    WB analysis using 10508-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10508-1-AP (SURVIVIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 10508-1-AP

    IHC staining of human colon cancer using 10508-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SURVIVIN antibody included
  • IHC staining of human colon cancer using 10508-1-AP

    IHC staining of human colon cancer using 10508-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SURVIVIN antibody included
  • IP experiment of U2OS using 10508-1-AP

    IP experiment of U2OS using 10508-1-AP

    IP result of anti-SURVIVIN (IP:10508-1-AP, 4ug; Detection:10508-1-AP 1:1000) with U2OS cells lysate 440 ug.

  • IF Staining of MCF-7 using 10508-1-AP

    IF Staining of MCF-7 using 10508-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SURVIVIN antibody (10508-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse testis using 10508-1-AP

    IF Staining of mouse testis using 10508-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SURVIVIN antibody (10508-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of Jurkat using 10508-1-AP

    FC experiment of Jurkat using 10508-1-AP

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug SURVIVIN Polyclonal antibody (10508-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

SURVIVIN Polyclonal antibody

SURVIVIN Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 10508-1-AP

产品说明书

引用文献(151)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (3)

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (2)

别名

BIRC5, IAP4, EPR 1, BIRC 5, Baculoviral IAP repeat-containing protein 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells, Jurkat cells, NIH/3T3 cells, A549 cells, MCF-7 cells, A431 cells, Ramos cells
Positive IP detected inU2OS cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue, human urothelial carcinoma tissue, human stomach cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy SURVIVIN Ready-To-Use IHC Kit. SURVIVIN primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10508-1-AP targets SURVIVIN in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat, canine, hamster
免疫原 SURVIVIN fusion protein Ag0788 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 baculoviral IAP repeat-containing 5
别名 BIRC5, IAP4, EPR 1, BIRC 5, Baculoviral IAP repeat-containing protein 5
计算分子量 16 kDa
观测分子量 16-18 kDa
GenBank蛋白编号BC008718
基因名称 Survivin
Gene ID (NCBI) 332
RRIDAB_2064048
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15392
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.

实验方案

Product Specific Protocols
WB protocol for SURVIVIN antibody 10508-1-APDownload protocol
IHC protocol for SURVIVIN antibody 10508-1-APDownload protocol
IF protocol for SURVIVIN antibody 10508-1-APDownload protocol
IP protocol for SURVIVIN antibody 10508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biomaterials

PMID: 29028548

Authors - Yudong Song
View Article
human,mouseWB

Theranostics

Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI

Authors - Bojun Li
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humanWB

Autophagy

Elaiophylin, a novel autophagy inhibitor, exerts antitumor activity as a single agent in ovarian cancer cells.

Authors - Xuejiao Zhao
View Article
humanWB

Circ Res

Iron Homeostasis and Pulmonary Hypertension: Iron Deficiency Leads to Pulmonary Vascular Remodeling in the Rat.

Authors - Emanuele Cotroneo
View Article
humanWB

J Exp Clin Cancer Res

ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK

Authors - Li Cui
View Article
humanWB

Cancer Lett

SKP2 promotes the metastasis of pancreatic ductal adenocarcinoma by suppressing TRIM21-mediated PSPC1 degradation

Authors - Jiahui Yuan
View Article