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JAK2 Polyclonal antibody

JAK2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17670-1-AP

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Synonyms

JAK 2, JAK2, Janus kinase 2, JTK10, Tyrosine protein kinase JAK2



经过测试的应用

Positive WB detected inJurkat cells, NIH/3T3 cells
Positive IP detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 17670-1-AP is JAK2 Fusion Protein expressed in E. coli.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig, zebrafish
Immunogen JAK2 fusion protein Ag11264 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Janus kinase 2 (a protein tyrosine kinase)
Synonyms JAK 2, JAK2, Janus kinase 2, JTK10, Tyrosine protein kinase JAK2
Calculated Molecular Weight 1132 aa, 130 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC039695
Gene Symbol JAK2
Gene ID (NCBI) 3717
RRIDAB_2811138
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60674
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Janus kinases (JAKs) are non-receptor tyrosine kinases that are activated by cytokine binding to cytokine receptors resulting in stimulation of the JAK-STAT signaling pathway. Aberrant activation of this signaling pathway is associated with numerous immune disorders and cancer. This antibody can detect full-length band of 130 kDa, and another bands 150 kDa after posttranslational modification.

实验方案

Product Specific Protocols
WB protocol for JAK2 antibody 17670-1-APDownload protocol
IHC protocol for JAK2 antibody 17670-1-APDownload protocol
IP protocol for JAK2 antibody 17670-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Neuroinflammation

Kynurenic acid blunts A1 astrocyte activation against neurodegeneration in HIV-associated neurocognitive disorders

Authors - Jingxian Lun
humanWB,IP

Cell Death Dis

4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer

Authors - Yanan Wang
mouseWB

Theranostics

Combination therapy with ropivacaine-loaded liposomes and nutrient deprivation for simultaneous cancer therapy and cancer pain relief.

Authors - Jiqian Zhang
humanWB

Arch Med Res

Extracellular Vesicles from Carcinoma-associated Fibroblasts Promote EMT of Salivary Adenoid Cystic Carcinoma Via IL-6

Authors - Hyat Ahmed Ibrahim Al-Raimi
mouseWB

Oxid Med Cell Longev

Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.

Authors - Wei Wang
ratIF

Brain Behav Immun

Interleukin-6: Its role and mechanisms in rescuing depression-like behaviors in rat models of depression.

Authors - Peng Wang