验证数据展示
经过测试的应用
| Positive WB detected in | PC-12 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rabbit cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue |
| Positive IHC detected in | mouse brain tissue, human rectal cancer tissue, rat pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse pancreas tissue, mouse brain tissue |
| Positive IF/ICC detected in | SH-SY5Y cells, PC-12 cells |
Planning an IHC experiment? We recommend our IHCeasy Synaptophysin Ready-To-Use IHC Kit. Synaptophysin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67864-1-Ig targets Synaptophysin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Synaptophysin fusion protein Ag11803 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | synaptophysin |
| 别名 | SYP, SYN, MRX96, 4E12C4 |
| 计算分子量 | 313 aa, 34 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC064550 |
| 基因名称 | Synaptophysin |
| Gene ID (NCBI) | 6855 |
| RRID | AB_2918622 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P08247 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Synaptophysin antibody 67864-1-Ig | Download protocol |
| IHC protocol for Synaptophysin antibody 67864-1-Ig | Download protocol |
| IF protocol for Synaptophysin antibody 67864-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Agric Food Chem 20(S)-Protopanaxadiol Exerts Antidepressive Effects in Chronic Corticosterone-Induced Rodent Animal Models as an Activator of Brain-Type Creatine Kinase | ||
Front Cell Neurosci Expression of SH3 and Multiple Ankyrin Repeat Domains Protein 3 in Mouse Retina. | ||
Psychiatry Res The role of reelin in the pathological mechanism of depression from clinical to rodents | ||
Alzheimers Dement Reduced synaptic proteins and SNARE complexes in Down syndrome with Alzheimer's disease and the Dp16 mouse Down syndrome model: Impact of APP gene dose | ||
Neurochem Int Photobiomodulation treatment inhibits neurotoxic astrocytic polarization and protects neurons in in vitro and in vivo stroke models | ||
J Agric Food Chem Protective Effect of 20(S)-Protopanaxadiol on D-Gal-Induced Cognitively Impaired Mice Based on Its Target Protein Brain-type Creatine Kinase |
