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CoraLite® Plus 647-conjugated Synaptophysin Monoclonal antibody

Synaptophysin Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat

Applications

IF-P

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4E12C4

Cat no : CL647-67864

Print datasheet

Synonyms

synaptophysin, SYP

验证数据展示

  • IF Staining of mouse brain using CL647-67864

    IF Staining of mouse brain using CL647-67864

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-67864, Clone: 4E12C4 ) at dilution of 1:200.

  • IF Staining of mouse brain using CL647-67864

    IF Staining of mouse brain using CL647-67864

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-67864, Clone: 4E12C4 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy Synaptophysin Ready-To-Use IHC Kit. Synaptophysin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67864-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67864 targets Synaptophysin in IF-P applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Applications IF-P Application Description
Tested Reactivity Human, Pig, Mouse, Rat
Immunogen Synaptophysin fusion protein Ag11803 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name synaptophysin
Synonyms synaptophysin, SYP
Calculated Molecular Weight 313 aa, 34 kDa
GenBank Accession NumberBC064550
Gene Symbol Synaptophysin
Gene ID (NCBI) 6855
RRIDAB_2920318
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08247
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Synaptophysin antibody CL647-67864Download protocol
Standard Protocols
Click here to view our Standard Protocols