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验证数据展示

  • IF Staining of mouse brain using CL488-67864

    IF Staining of mouse brain using CL488-67864

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Synaptophysin antibody (CL488-67864, Clone: 4E12C4 ) at dilution of 1:200.

  • IF Staining of mouse brain using CL488-67864

    IF Staining of mouse brain using CL488-67864

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Synaptophysin antibody (CL488-67864, Clone: 4E12C4 ) at dilution of 1:200.

  • FC experiment of PC-12 using CL488-67864

    FC experiment of PC-12 using CL488-67864

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Synaptophysin (CL488-67864, Clone:4E12C4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated Synaptophysin Monoclonal antibody

Synaptophysin Monoclonal Antibody for IF-P, FC (Intra)
Cat No. CL488-67864

产品说明书

CloneNo. 4E12C4
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF-P, FC (Intra) and More (1)

别名

SYP, SYN, MRX96, 4E12C4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inPC-12 cells
Planning an IHC experiment? We recommend our IHCeasy Synaptophysin Ready-To-Use IHC Kit. Synaptophysin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67864-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-67864 targets Synaptophysin in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF-P, FC (Intra) Application Description
文献引用应用IF
经测试反应性 human, mouse, rat, pig
文献引用反应性human
免疫原 Synaptophysin fusion protein Ag11803 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 synaptophysin
别名 SYP, SYN, MRX96, 4E12C4
计算分子量 313 aa, 34 kDa
GenBank蛋白编号BC064550
基因名称 Synaptophysin
Gene ID (NCBI) 6855
RRIDAB_2909472
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP08247
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Synaptophysin antibody CL488-67864Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Int J Mol Sci

Morphological Evidence for a Unique Neuromuscular Functional Unit of the Human Vocalis Muscle

Authors - Rareș-Vasile Tracicaru
View Article