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验证数据展示

  • IF Staining of mouse testis using 67982-1-Ig

    IF Staining of mouse testis using 67982-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using TEX14 antibody (67982-1-Ig, Clone: 1A11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse testis using 67982-1-Ig

    IF Staining of mouse testis using 67982-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using TEX14 antibody (67982-1-Ig, Clone: 1A11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis of rat testis using 67982-1-Ig

    WB analysis of rat testis using 67982-1-Ig

    rat testis tissue were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

TEX14 Monoclonal antibody

TEX14 Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 67982-1-Ig

产品说明书

CloneNo. 1A11D6

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat, rabbit

应用

WB, IF-P, ELISA

别名

SGK307, SGK307, Sugen kinase 307, testis expressed 14, Testis expressed protein 14, Testis expressed sequence 14, TEX14

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat testis tissue, PC-3 cells, mouse testis tissue, rabbit testis tissue
Positive IF-P detected inmouse testis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67982-1-Ig targets TEX14 in WB, IF-P, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 Human, mouse, rat, rabbit
免疫原 TEX14 fusion protein Ag13143 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 testis expressed 14
别名 SGK307, SGK307, Sugen kinase 307, testis expressed 14, Testis expressed protein 14, Testis expressed sequence 14, TEX14
计算分子量957aa,107 kDa; 1497aa,168 kDa
观测分子量180 kDa
GenBank蛋白编号BC040526
基因名称 TEX14
Gene ID (NCBI) 56155
RRIDAB_2918731
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IWB6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tex14 is required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Tex14 acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges.Tex14 also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment.

实验方案

Product Specific Protocols
WB protocol for TEX14 antibody 67982-1-IgDownload protocol
IF protocol for TEX14 antibody 67982-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols