验证数据展示
经过测试的应用
Positive IF-P detected in | mouse testis tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67982 targets TEX14 in IF-P applications and shows reactivity with Human, mouse, rat, rabbit samples.
经测试应用 | IF-P Application Description |
经测试反应性 | Human, mouse, rat, rabbit |
免疫原 | TEX14 fusion protein Ag13143 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | testis expressed 14 |
别名 | SGK307, SGK307, Sugen kinase 307, testis expressed 14, Testis expressed protein 14, Testis expressed sequence 14, TEX14 |
计算分子量 | 957aa,107 kDa; 1497aa,168 kDa |
观测分子量 | 180 kDa |
GenBank蛋白编号 | BC040526 |
基因名称 | TEX14 |
Gene ID (NCBI) | 56155 |
RRID | AB_2923849 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q8IWB6 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tex14 is required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Tex14 acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges.Tex14 also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 TEX14 antibody CL488-67982 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |