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CoraLite®594-conjugated TEX14 Monoclonal antibody

TEX14 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, rabbit

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A11D6

Cat no : CL594-67982

Print datasheet

Synonyms

SGK307, SGK307, Sugen kinase 307, testis expressed 14, Testis expressed protein 14, Testis expressed sequence 14, TEX14

验证数据展示

  • IF Staining of mouse testis using CL594-67982

    IF Staining of mouse testis using CL594-67982

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 TEX14 antibody (CL594-67982, Clone: 1A11D6 ) at dilution of 1:200.

  • IF Staining of mouse testis using CL594-67982

    IF Staining of mouse testis using CL594-67982

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 TEX14 antibody (CL594-67982, Clone: 1A11D6 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inmouse testis tissue
For other applications, we recommend the unconjugated version of this antibody, 67982-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67982 targets TEX14 in IF-P applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Applications IF-P Application Description
Tested Reactivity Human, mouse, rat, rabbit
Immunogen TEX14 fusion protein Ag13143 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name testis expressed 14
Synonyms SGK307, SGK307, Sugen kinase 307, testis expressed 14, Testis expressed protein 14, Testis expressed sequence 14, TEX14
Calculated Molecular Weight957aa,107 kDa; 1497aa,168 kDa
Observed Molecular Weight180 kDa
GenBank Accession NumberBC040526
Gene Symbol TEX14
Gene ID (NCBI) 56155
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IWB6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tex14 is required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Tex14 acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges.Tex14 also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment.

实验方案

Product Specific Protocols
IF protocol for CL594 TEX14 antibody CL594-67982Download protocol
Standard Protocols
Click here to view our Standard Protocols