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ChromoTek TurboGFP-Trap Magnetic Agarose, kit

TurboGFP-Trap Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of TurboGFP fusion proteins. It consists of an anti-TurboGFP VHH/ Nanobody that is coupled to magnetic agarose beads.

Specificity

TurboGFP, CopGFP, maxGFP

Applications

IP, CoIP

Type

Nanobody

Conjugate

Magnetic agarose

Cat No : tbtmak

Synonyms

TurboGFP-Trap, TurboGFP, CopGFP, maxGFP,maxFP-Green, green fluorescent protein CopGFP, TurboGFP tag

Validation Data Gallery

  • Coomassie blue stained gel and Western blot.

    Coomassie blue stained gel and Western blot.

  • TurboGFP-Trap Magnetic Agarose for pull-down of TurboGFP fusion proteins: Coomassie and Western Blot Input (I), non-bound (FT), and bound (B).

    TurboGFP-Trap Magnetic Agarose for pull-down of TurboGFP fusion proteins: Coomassie and Western Blot Input (I), non-bound (FT), and bound (B).

Product Information

TurboGFP-Trap Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of TurboGFP fusion proteins. It consists of an anti-TurboGFP VHH/ Nanobody that is coupled to magnetic agarose beads.

DescriptionThe TurboGFP-Trap® Magnetic Agarose Kit contains TurboGFP-Trap Magnetic Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of TurboGFP-fusion proteins and their interacting factors.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains on the SDS-PAGE

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP
Specificity/TargetTurboGFP, CopGFP, maxGFP, maxFP-Green
For the complete list, please click here: Fluorescent protein specificity table
Binding capacity7.5-10 μg of recombinant TurboGFP per 25 μL bead slurry
ConjugateMagnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
Elution bufferSDS sample buffer
0.1 mM citrate acid pH 3.0
Wash buffer compatibility6 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 0.5 nM
Compatibility with mass spectrometryThe TurboGFP-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2827597
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

ComponentDescription
TurboGFP-Trap Magnetic Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution

Publications

ApplicationTitle

Plant Biotechnol J

Fine-tuning the N-glycosylation of recombinant human erythropoietin using Chlamydomonas reinhardtii mutants

Authors - S Leprovost
View Article