• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Validation Data Gallery

  • Coomassie blue stained gel and Western blot.

    Coomassie blue stained gel and Western blot.

  • TurboGFP-Trap Magnetic Agarose for pull-down of TurboGFP fusion proteins: Coomassie and Western Blot Input (I), non-bound (FT), and bound (B).

    TurboGFP-Trap Magnetic Agarose for pull-down of TurboGFP fusion proteins: Coomassie and Western Blot Input (I), non-bound (FT), and bound (B).

ChromoTek TurboGFP-Trap Magnetic Agarose

TurboGFP-Trap Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of TurboGFP fusion proteins.
Cat No. tbtma
引用文献(3)

Specificity

TurboGFP, CopGFP, maxGFP

Applications

IP, CoIP

Type

Nanobody

Conjugate

Magnetic agarose

Synonyms

TurboGFP-Trap, TurboGFP, CopGFP, maxGFP,maxFP-Green, green fluorescent protein CopGFP, TurboGFP tag

Formats:  Magnetic Agarose
规格价格库存

点击咨询售前客服

点击咨询售后客服

Product Information

TurboGFP-Trap Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of TurboGFP fusion proteins.

DescriptionImmunoprecipitation of TurboGFP-fusion proteins and their interacting factors with anti-TurboGFP Nanobody conjugated to magnetic agarose beads.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

• Works in samples from: mammals, plants, bacteria, yeast, insects etc.

ApplicationsIP, CoIP
Specificity/TargetTurboGFP, CopGFP, maxGFP, maxFP-Green
For the complete list, please click here: Fluorescent protein specificity table
Binding capacity7.5-10 μg of recombinant TurboGFP per 25 μL bead slurry
ConjugateMagnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
Elution bufferSDS sample buffer
0.1 mM citrate acid pH 3.0
Wash buffer compatibility6 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 0.5 nM
Compatibility with mass spectrometryThe TurboGFP-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2827597
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!
Size25ul/reactions (eg：20rxns=500ul slurry)

Publications

ApplicationTitle
CoIP

Mol Biol Cell

RUSC2 and WDR47 oppositely regulate kinesin-1-dependent distribution of ATG9A to the cell periphery.

Authors - Carlos M Guardia
View Article
IP

Front Immunol

SIRT2-PFKP interaction dysregulates phagocytosis in macrophages with acute ethanol-exposure

Authors - Anugraha Gandhirajan
View Article
CoIP

Development

CHD4 and SMYD1 repress common transcriptional programs in the developing heart

Authors - Wei Shi
View Article