Validation Data Gallery
Product Information
TurboGFP-Trap Agarose is an affinity bead for immunoprecipitation (IP) of TurboGFP fusion proteins It comprises an anti-TurboGFP VHH/ Nanobody coupled to agarose beads.
| Description | Immunoprecipitation of TurboGFP-fusion proteins and their interacting factors with anti-TurboGFP Nanobody conjugated to beads.
• Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis • Works in samples from: mammals, plants, bacteria, yeast, insects etc. |
| Applications | IP, CoIP |
| Specificity/Target | TurboGFP, CopGFP, maxGFP, maxFP-Green For the complete list, please click here: Fluorescent protein specificity table |
| Binding capacity | 7.5-10 μg of recombinant TurboGFP per 25 μL bead slurry |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.1 mM citrate acid pH 3.0 |
| Wash buffer compatibility | 6 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 0.5 nM |
| Compatibility with mass spectrometry | The TurboGFP-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2827596 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
| Size | 25ul/reactions (eg：20rxns=500ul slurry) |
Documentation
| SDS |
|---|
| tbta_SDS_TurboGFP-Trap Agarose (EN) |
| Datasheet |
|---|
| Protocol TurboGFP-Trap Agarose (PDF) |
| Troubleshooting |
|---|
| Troubleshooting guide immunoprecipitation (PDF) |
| Specificity |
|---|
| Fluorescent protein specificity table (PDF) |
Publications
| Application | Title |
|---|---|
Mol Cancer Tumour-intrinsic PDL1 signals regulate the Chk2 DNA damage response in cancer cells and mediate resistance to Chk1 inhibitors | |
Mol Metab The pseudokinase TRIB3 controls adipocyte lipid homeostasis and proliferation in vitro and in vivo | |
Cancers (Basel) Comprehensive Profiling of Mammalian Tribbles Interactomes Implicates TRIB3 in Gene Repression. | |
Front Cell Dev Biol Modulation of Increased mGluR1 Signaling by RGS8 Protects Purkinje Cells From Dendritic Reduction and Could Be a Common Mechanism in Diverse Forms of Spinocerebellar Ataxia. |